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KMID : 0380020190340020099
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 2 p.99 ~ p.106
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Anti-inflammatory effect of Sechium edule extract in LPS-stimulated RAW 264.7 cells via p-JNK and p-p38 down-regulation
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Kim Min-Jin
Chung You-Chul
Kim Sang-Suk
Lim Chan-Kyu
Park Kyung-Jin
Choi Young-Hun
Park Tae-Jin
Kim Seung-Young
Hyun Chang-Gu
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Abstract
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The macrophages, known as major cells involved in inflammatory response, are activated by immune cell-producing cytokine as well as by several stimuli to produce proinflammatory cytokine, and nitric oxide (NO), prostaglandin E2 (PGE2) with are related with inducinle NOS (iNOS) and cyclooxygenase-2 (COX-2). In this study, the anti-inflammatory activity and non-toxicity of Sechium edule extract (SEE) were tested by measuring the expression level of NO, PGE2, and pro-inflammatory cytokines (interleukin-1β, interleukin-6, tumor necrosis factor-α) in lipopolysaccharide (LPS) and Sechium edule extract-treated in RAW 264.7 macrophages. Sechium edule extract inhibited the production of NO, PGE2, and pro-inflammatory cytokines in a dose-dependent manner but without compromising cell viability. In addition, we confirmed that the inhibition activity of Sechium edule extract on the production of NO and PEG2 was via the down-regulation of iNOS and COX-2, respectively. Moreover, expression levels of the phosphorylation of Mitogen-activated protein kinase (MAP kinase) family such as c-Jun N-termical kinases (JNK) and p38 were reduced by Sechium edule extract. Our results clearly indicate that Sechium edule extract can be a potential new source for anti-inflammatory drugs and healthcare product ingredients.
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KEYWORD
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Sechium edule, lipopolysaccharide, iNOS, COX-2
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